EB05314 Goat Anti-RAC2 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100ug specific antibody in 200ul
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: RAC2; ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2); EN-7; Ras-related C3 botulinum toxin substrate 2; rho family, small GTP binding protein Rac2; Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP-binding protein Rac2)
Official Symbol: RAC2
Accession Number(s): NP_002863.1
Human GeneID(s): 5880
Gene Ontology Terms: Rho small monomeric GTPase activity; small GTPase mediated signal transduction; signal transduction
Immunogen Peptide with sequence PQPTRQQKRACSLL, from the C Terminus of the protein sequence according to NP_002863.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 22kDa band observed in lysates of Human T cell line MOLT4 (calculated MW of 21.4kDa according to NP_002863.1). Recommended concentration: 1-3µg/ml.
Immunohistochemistry: In paraffin embedded Human Tonsil, stains most cells in the mantle zone and interfollicular area. In the germinal centres the majority of cells are negative. Recommended concentration 0.3ug/mlSpecies ReactivityTested: Human
Expected from sequence similarity: Human, Mouse, Rat
Background ReferenceReibel L, Dorseuil O, Stancou R, Bertoglio J, Gacon G.
A hemopoietic specific gene encoding a small GTP binding protein is overexpressed during T cell activation.
Biochem Biophys Res Commun. 1991 Mar 15;175(2):451-8.
PMID: 1902092
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB05314 staining (2µg/ml) of MOLT-4 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05314 (0.3µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Strong staining in the interfollicular area and mantle zone. B) Positive cells in the interfollicular area. |
