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Code Name Applications Availability Size Tested species Price Grade  
EB13027 Goat Anti-MDH2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $411.00 Elite

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$73.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB13027 1
EB13027 (0.1µg/ml) staining of HeLa (A), HepG2 (B) and NIH3T3 (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB13027 2
EB13027 (0.01µg/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB13027 3
EB13027 (5µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: MDH2, malate dehydrogenase 2, NAD (mitochondrial), M-MDH, MDH, MGC:3559, MOR1, malate dehydrogenase, mitochondrial
Official Symbol: MDH2
Accession Number(s): NP_005909.2
Human GeneID(s): 4191
Non-Human GeneID(s): 17448 (mouse), 81829 (rat)

Immunogen

Peptide with sequence C-TRDDLFNTNAT, from the internal region of the protein sequence according to NP_005909.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 35kDa band observed in lysates of cell lines HeLa, HepG2 and NIH3T3 (calculated MW of 35.5kDa according to Human NP_005909.2 and 35.6kDa according to Mouse NP_032643.2 ). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. Approx 35kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 35.5kDa according to Human NP_005909.2 and 35.6kDa according to Mouse NP_032643.2, Rat NP_112413.2 and Pig NP_001231082.1 ). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

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