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Code Name Applications Availability Size Tested species Price Grade  
EB12283 Goat Anti-NR5A2 / LRH1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Pig $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12283 1
EB12283 (1µg/ml) staining of HepG2 nuclear lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12283 2
EB12283 (0.3µg/ml) staining of Pig Ovary lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: NR5A2, nuclear receptor subfamily 5, group A, member 2, B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2, CYP7A promoter-binding factor, alpha-1-fetoprotein transcription factor, b1-binding factor, hepatocyte transcription factor which activates enhancer II of hepatitis B virus, fetoprotein-alpha 1 (AFP) transcription factor, hepatocytic transcription factor, liver nuclear receptor homolog-1 variant 2, liver receptor homolog 1, liver receptor homolog-1, nuclear receptor NR5A2, nuclear receptor subfamily 5 group A member 2
Official Symbol: NR5A2
Accession Number(s): NP_995582.1; NP_003813.1
Human GeneID(s): 2494
Non-Human GeneID(s): 26424 (mouse), 60349 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_995582.1; NP_003813.1).

Immunogen

Peptide with sequence C-TDYDRSPFVTSPIS, from the internal region of the protein sequence according to NP_995582.1; NP_003813.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 55kDa band observed in nuclear lysates of cell lines HeLa and HepG2, and approx 60kDa band observed in Pig Ovary lysates (calculated MW of 56.5kDa according to Human NP_003813.1 and Pig AFD98842.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Liver and NIH-3T3 cell line for this particular batch.

Species Reactivity

Tested: Human, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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