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Goat Anti-Lipin 3 Antibody (EB07456)

Related Products: EBP07456 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07456 Goat Anti-Lipin 3 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB07456 1
EB07456 (2.5µg/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: LPIN3, lipin 3, RP4-620E11.3, LIPN3L, SMP2, dJ450M14.2, dJ450M14.3, dJ620E11.2, lipin 3-like
Official Symbol: LPIN3
Accession Number(s): NP_075047.1
Human GeneID(s): 64900

Immunogen

Peptide with sequence C-KPKQKEDAVATD, from the internal region of the protein sequence according to NP_075047.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave bands at approx 80kDa and 60kDa in Human Liver lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 93.6kDa according to NP_075047.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Thyroid Gland shows staining of cytoplasm and cell membrane of epithelial cells surrounding the follicles. Recommended concentration, 2-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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