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Functional Clustering

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  • Secondary Antibodies

  • Rabbit anti-Goat HRP Conjugated


  • Peptides

  • Immunising Peptides available for all antibodies
  • Everest Biotech Ltd Goat Anti-Ezrin / villin 2 Antibody
    Online Catalogue > 14-3-3 sigma to FZD9 > Goat Anti-Ezrin / villin 2 Antibody

    EB07305-T Goat Anti-Ezrin / villin 2 Antibody - Trial Size
    Price: $100.00 / €100,00 / £75.00
    Size: 20µg specific antibody in 40µl
    EU Availability: In stock
    US Availability: In stock
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    EB07305 Goat Anti-Ezrin / villin 2 Antibody
    Price: $285.00 / €292,50 / £195.00
    Size: 100µg specific antibody in 200µl
    EU Availability: In stock
    US Availability: In stock
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    Cart for UK (GBP) customers coming soon, meanwhile click for ordering info

    Ordering Information

    As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.

    Target Protein

    Principal Names: villin 2 (ezrin) ; ezrin; VIL2; CVIL; CVL; DKFZp762H157; FLJ26216; MGC1584 ; cytovillin; villin 2
    Official Symbol: VIL2
    Accession Number(s): NP_003370.2
    Human GeneID(s): 7430
    Non-Human GeneID(s): 22350 (mouse); 54319 (rat);

    Immunogen

    Peptide with sequence C-QDYEEKTKKAER, from the internal region of the protein sequence according to NP_003370.2.

    Purification + Storage

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    Aliquot and store at -20°C. Minimize freezing and thawing.

    Applications Tested

    Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 80kDa band observed in Human Duodenum and Placenta lysates (calculated MW of 69.4kDa according to NP_003370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhu et al, Am J Physiol Cell Physiol. 2005 Jun;288(6):C1242-54.; PMID: 15788482). Recommended concentration: 1-3µg/ml.

    Species Reactivity

    Tested: Human
    Expected from sequence similarity: Human, Dog

    Background Reference

    Stanasila L, Abuin L, Diviani D, Cotecchia S.
    Ezrin directly interacts with the alpha1b-adrenergic receptor and plays a role in receptor recycling.
    J Biol Chem. 2006 Feb 17;281(7):4354-63. Epub 2005 Dec 13.
    PMID: 16352594
     


    Adobe PDF Version of Datasheet


    For Research purposes only. Not for diagnostic or in vivo use.

    © Copyright Everest Biotech Ltd 2009

    Elite Grade Product

    Elite Grade Antibodies

    Trial Size Available


    EB07305 Goat Anti-Ezrin / villin 2 Antibody

    EB07305 (1µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

    Online Catalogue > 14-3-3 sigma to FZD9 > Goat Anti-Ezrin / villin 2 Antibody