EB07268 Goat Anti-NET1 / ARHGEF8 (Internal) Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: NET1; ARHGEF8; neuroepithelial cell transforming gene 1; NET1A; Rho guanine nucleotide exchange factor (GEF) 8; guanine nucleotide regulatory protein (oncogene); neuroepithelioma transforming gene 1; p65 Net1 proto-oncogene protein; small GTP-binding protein regulator
Official Symbol: NET1
Accession Number(s): NP_005854.2; NP_001040625.1
Human GeneID(s): 10276
Non-Human GeneID(s): 56349 (mouse); 307098 (rat);
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2). Immunogen Peptide with sequence C-DKLEYLDEKQRDPR, from the internal region of the protein sequence according to NP_005854.2; NP_001040625.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave an approx 80-90kDa double band in Human Placenta lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.7kDa according to NP_001040625.1. The 80-90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Immunohistochemistry: In paraffin embedded Human Breast shows cytoplasm staining of myoepithelial cells of ducts. Recommended concentration, 2.5µg/ml.Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog
Background ReferenceQin H, Carr HS, Wu X, Muallem D, Tran NH, Frost JA.
Characterization of the biochemical and transforming properties of the neuroepithelial transforming protein 1.
J Biol Chem. 2005 Mar 4;280(9):7603-13. Epub 2004 Dec 16.
PMID: 15611121
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For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB07268 (2.5µg/ml) staining of paraffin embedded Human Breat. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
