EB07246 Goat Anti-NONO / p54NRB Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: NONO; non-POU domain containing, octamer-binding ; HGNC:7871; NMT55; NRB54; P54; P54NRB ; Nuclear RNA-binding protein, 54-kD; non-POU-domain-containing, octamer-binding; non-Pou domain-containing octamer (ATGCAAAT) binding protein Official Symbol: NONO Accession Number(s): NP_031389.3 Human GeneID(s): 4841 Non-Human GeneID(s): 53610 (mouse); 317259 (rat); Immunogen Peptide with sequence C-NRAAPGAEFAPNK, from the C Terminus of the protein sequence according to NP_031389.3. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 60kDa band observed in mouse brain lysates (calculated MW of 54.2kDa according to NP_031389.3). Recommended concentration: 0.1-0.3µg/ml.
Species ReactivityTested: Mouse Expected from sequence similarity: Human, Mouse, Rat, Dog Background ReferenceBrown SA, Ripperger J, Kadener S, Fleury-Olela F, Vilbois F, Rosbash M, Schibler U. PERIOD1-associated proteins modulate the negative limb of the mammalian circadian oscillator. Science. 2005 Apr 29;308(5722):693-6. PMID: 15860628
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For Research purposes only. Not for diagnostic or in vivo use. © Copyright Everest Biotech Ltd 2009 | Elite Grade Product 

EB07246 (0.1µg/ml) staining of mouse brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |