EB07137 Goat Anti-GLI3 Antibody
Price: $185.00 / €187,50 / £125.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: GLI3; GLI-Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome) ; ACLS; GCPS; PAP-A; PAPA; PAPA1; PAPB; PHS; PPDIV ; DNA-binding protein; GLI-Kruppel family member GLI3; oncogene GLI3; zinc finger protein GLI3
Official Symbol: GLI3
Accession Number(s): NP_000159.2
Human GeneID(s): 2737
Gene Ontology Terms: SHH signalling, Sonic Hedgehog signalling pathway
Immunogen Peptide with sequence C-HNKRSKIKPDED, from the internal region of the protein sequence according to NP_000159.2. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments in Human Brain, Lung, Placenta and Testes lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Species ReactivityTested:
Expected from sequence similarity: Human, Mouse, Rat, Dog
Background ReferenceHaycraft CJ, Banizs B, Aydin-Son Y, Zhang Q, Michaud EJ, Yoder BK.
Gli2 and gli3 localize to cilia and require the intraflagellar transport protein polaris for processing and function.
PLoS Genet. 2005 Oct;1(4):e53. Epub 2005 Oct 28.
PMID: 16254602
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Aspiring Grade Product 
Please note, this product is one of a range of Aspiring Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Application Notes below). However no additional claims are made for their ability to recognise native protein in any application. |
