EB07136 Goat Anti-GLI2 Antibody
Price: $185.00 / €187,50 / £125.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: GLI2; GLI-Kruppel family member GLI2 ; THP2; oncogene GLI2; tax helper protein 2; tax-responsive element-2 holding protein; tax-responsive element-25-bp sequence binding protein; zinc finger protein GLI2
Official Symbol: GLI2
Accession Number(s): NP_005261.2
Human GeneID(s): 2736
Gene Ontology Terms: SHH signalling, Sonic Hedgehog signalling pathway
Important Comments: This antibody is expected to recognise all four reported isoforms (NP_084655.1, NP_084656.1, NP_084657.1 and NP_005261.1) Immunogen Peptide with sequence DLKEDLDRDDCKQE, from the internal region of the protein sequence according to NP_005261.2. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:2,000. Western Blot: Preliminary experiments gave an approx 55kDa band in Human Bone Marrow and Ovary lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 168kDa according to NP_005261.2. The 55kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Species ReactivityTested:
Expected from sequence similarity: Human, Mouse
Background ReferenceHaycraft CJ, Banizs B, Aydin-Son Y, Zhang Q, Michaud EJ, Yoder BK.
Gli2 and gli3 localize to cilia and require the intraflagellar transport protein polaris for processing and function.
PLoS Genet. 2005 Oct;1(4):e53. Epub 2005 Oct 28.
PMID: 16254602
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Aspiring Grade Product 
Please note, this product is one of a range of Aspiring Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Application Notes below). However no additional claims are made for their ability to recognise native protein in any application. |
