EB07125 Goat Anti-DCP1A Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: DCP1A; decapping enzyme ; HSA275986; Nbla00360; SMAD4IP1; SMIF; Smad4-interacting transcriptional co-activator; decapping enzyme hDcp1a; putative protein product of Nbla00360; transcription factor SMIF
Official Symbol: DCP1A
Accession Number(s): NP_060873.3
Human GeneID(s): 55802
Non-Human GeneID(s): 75901 (mouse);
Immunogen Peptide with sequence C-QVLTKNKDNHN, from the C Terminus of the protein sequence according to NP_060873.3. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 75kDa band observed in human lysates of A431 and HeLa (calculated MW of 63.3kDa according to NP_060873.3). Recommended concentration: 1-3µg/ml. An additional band of 48kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse, Rat
Background ReferenceCougot N, Babajko S, Seraphin B.
Cytoplasmic foci are sites of mRNA decay in human cells.
J Cell Biol. 2004 Apr;165(1):31-40. Epub 2004 Apr 5.
PMID: 15067023
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB07125 (2µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
