EB07121 Goat Anti-ADRA2A Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
Click to add to EURO Shopping Cart (EU customers only)
Click to add to US Dollar Shopping Cart (US customers only)
Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: ADRA2A; adrenergic, alpha-2A-, receptor ; ADRA2; ADRA2R; ADRAR; ALPHA2AAR; ZNF32 ; OTTHUMP00000058849; alpha-2A-adrenergic receptor; alpha-2AAR subtype C10
Official Symbol: ADRA2A
Accession Number(s): NP_000672.2
Human GeneID(s): 150
Immunogen Peptide with sequence C-TERRPNGLGPERS, from the internal region of the protein sequence according to NP_000672.2. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave a band at approx 37kDa and a minor band at approx 23kDa in Human Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 49.0kDa according to NP_000672.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
This product has been successfully used in Immunofluorescense on Mouse primary DRG cultured neurons: Tan et al, J Biol Chem. 2009 Mar 6;284(10):6270-81 (PMID: 19126537).Species ReactivityTested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog
Product Specific ReferenceThe goat polyclonal antibody used in this paper was manufactured by us:
Tan M. et al.: p38 MAPK and beta-arrestin 2 mediate functional interactions between endogenous micro-opioid and alpha2A-adrenergic receptors in neurons. J Biol Chem. 2009 Mar 6;284(10):6270-81. Epub 2009 Jan 6.
PMID: 19126537
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
|
