EB07081 Goat Anti-ERG Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
Click to add to EURO Shopping Cart (EU customers only)
Click to add to US Dollar Shopping Cart (US customers only)
Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: ERG; v-ets erythroblastosis virus E26 oncogene like (avian) ; HGNC:3446; erg-3; p55; transcriptional regulator ERG (transforming protein ERG); v-ets avian erythroblastosis virus E26 oncogene related; v-ets erythroblastosis virus E26 oncogene like
Official Symbol: ERG
Accession Number(s): NP_891548.1; NP_004440.1
Human GeneID(s): 2078
Non-Human GeneID(s): 13876 (mouse); 170909 (rat);
Important Comments: This antibody is expected to recognise both reported isoforms (NP_891548.1; NP_004440.1) Immunogen Peptide with sequence C-PNTRLPTSHMPSH, from the C Terminus of the protein sequence according to NP_891548.1; NP_004440.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments in lysates of cell lines HEK293, A431 and Jurkat gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Immunohistochemistry: In paraffin embedded Prostate shows nucleolar staining in some of the epithelial cells. Recommended concentration, 10-20µg/ml.Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse, Rat
Background ReferenceTomlins SA, Rhodes DR, Perner S, Dhanasekaran SM, Mehra R, Sun XW, Varambally S, Cao X, Tchinda J, Kuefer R, Lee C, Montie JE, Shah RB, Pienta KJ, Rubin MA, Chinnaiyan AM.
Recurrent fusion of TMPRSS2 and ETS transcription factor genes in prostate cancer.
Science. 2005 Oct 28;310(5748):644-8.
PMID: 16254181
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB07081 (10µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
