EB07075 Goat Anti-RENT2 / UPF2 Antibody
Price: $185.00 / €187,50 / £125.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
Click to add to EURO Shopping Cart (EU customers only)
Click to add to US Dollar Shopping Cart (US customers only)
Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: UPF2; UPF2 regulator of nonsense transcripts homolog (yeast); HGNC:17854; DKFZP434D222; HUPF2; KIAA1408; RENT2 ; OTTHUMP00000045025; UPF2 regulator of nonsense transcripts homolog; regulator of nonsense transcripts 2; yeast Upf2p homolog
Official Symbol: UPF2
Accession Number(s): NP_542166.1; NP_056357.1
Human GeneID(s): 26019
Important Comments: This antibody is expected to recognise both reported isoforms (NP_542166.1; NP_056357.1). Immunogen Peptide with sequence C-PRYQHPKGAPNAD, from the C Terminus of the protein sequence according to NP_542166.1; NP_056357.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Preliminary experiments in Human HeLa lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Species ReactivityTested:
Expected from sequence similarity: Human, Mouse, Dog
Background ReferenceHosoda N, Kim YK, Lejeune F, Maquat LE.
CBP80 promotes interaction of Upf1 with Upf2 during nonsense-mediated mRNA decay in mammalian cells.
Nat Struct Mol Biol. 2005 Oct;12(10):893-901. Epub 2005 Sep 25.
PMID: 16186820
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Aspiring Grade Product 
Please note, this product is one of a range of Aspiring Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Application Notes below). However no additional claims are made for their ability to recognise native protein in any application. |
