EB07061 Goat Anti-14-3-3 sigma / Stratifin Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100ug specific antibody in 200ul
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: 14-3-3sigma; stratifin; HGNC:10773 ; SFN
Official Symbol: SFN
Accession Number(s): NP_006133.1
Human GeneID(s): 2810
Immunogen Peptide with sequence C-DLHTLSEDSYKDST, from the internal region of the protein sequence according to NP_006133.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx. 28kDa band observed in Human Skin lysates (calculated MW of 27.8kDa according to NP_006133.1). Recommended concentration: 0.1-0.3µg/ml.
Immunohistochemistry: In paraffin embedded Human Skin shows strong nuclear staining in selected cells of the basal layer . Recommended concentration, 2-3µg/ml.Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog
Background ReferenceLodygin D, Hermeking H.
The role of epigenetic inactivation of 14-3-3sigma in human cancer.
Cell Res. 2005 Apr;15(4):237-46. Review.
PMID: 15857578
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB07061 (0.1µg/ml) staining of human skin lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07061 (2.5µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
