EB07046 Goat Anti-PDE4D Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100ug specific antibody in 200ul
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: PDE4D; phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) ; HGNC:8783; DPDE3; HSPDE4D; PDE4DN2; STRK1; cAMP-specific phosphodiesterase 4D; cAMP-specific phosphodiesterase PDE4D6; dunce-like phosphodiesterase E3; phosphodiesterase 4D, cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase E3)
Official Symbol: PDE4D
Accession Number(s): NP_006194.2
Human GeneID(s): 5144
Important Comments: Please note this antibody is not expected to recognize isoform 4. Immunogen Peptide with sequence QPEACVIDDRSPDT, from the C Terminus of the protein sequence according to NP_006194.2. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Figure 1: detects transfected Human PDE4D1 and PDE4D3 in COS cell lysates as bands of approx. 68kDa and 95kDa respectively. Figure 2: Approx 70,+80+90kDa band observed in A549 lysates (calculated MW of 76.5kDa according to NP_006194.2). Recommended concentration: 0.1-0.3µg/ml.Recommended concentration: 1-3 µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human
Background ReferenceHouslay MD.
The long and short of vascular smooth muscle phosphodiesterase-4 as a putative therapeutic target.
Mol Pharmacol. 2005 Sep;68(3):563-7.
PMID: 15958394
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB07046 staining (1µg/ml) of COS cell lysates (25µg protein): transfected with Human PDE4D1 (1), transfected with Human PDE4D3 (2), untransfected (3). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07046 staining (0.3µg/ml) of A549 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
