EB06940 Goat Anti-Duffy / FY / DARC Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100ug specific antibody in 200ul
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: FY; DARC; HGNC:4035; Duffy blood group; CCBP1; GPD; CTA-134P22.3; CD234; FY; OTTHUMP00000060081; Duffy blood group, chemokine receptor; Duffy blood group antigen; Glycoprotein D
Official Symbol: DARC
Accession Number(s): NP_002027.2
Human GeneID(s): 2532
Immunogen Peptide with sequence HRAELSPSTENSSQLDFED-C, from the N Terminus of the protein sequence according to NP_002027.2. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 35kDa band observed in human kidney and human liver lysates (calculated MW of 35.6kDa according to NP_002027.2). Recommended concentration: 0.1-0.3µg/ml.
Immunohistochemistry: In paraffin embedded human kidney, stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. This antibody has also been successfully used on Huamn Lung showing endothelial staining. Recommended concentration, 3µg/ml.
FACS: A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.Species ReactivityTested: Human
Expected from sequence similarity: Human
Background ReferenceBruhl H, Vielhauer V, Weiss M, Mack M, Schlondorff D, Segerer S.
Expression of DARC, CXCR3 and CCR5 in giant cell arteritis.
Rheumatology (Oxford). 2005 Mar;44(3):309-13. Epub 2004 Nov 30.
PMID: 15572394
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06940 (0.1µg/ml) staining of human liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06940 (3µg/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. |
