EB06787 Goat Anti-Serotonin receptor 1A / HTR1A Antibody
Price: $185.00 / €187,50 / £125.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: HTR1A; 5-hydroxytryptamine (serotonin) receptor 1A ; HGNC:5286; 5-HT1A; ADRB2RL1; ADRBRL1; 5-HT1a receptor; G protein coupled receptor; guanine nucleotide-binding regulatory protein-coupled receptor
Official Symbol: HTR1A
Accession Number(s): NP_000515.2
Human GeneID(s): 3350
Important Comments: No crossreactivity expected with other serotonin receptors Immunogen Peptide with sequence C-KTVKKVEKTGADT, from the internal region of the protein sequence according to NP_000515.2. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave bands at approx 35kDa and 17kDa in lysates of Human Burkitt's Lymphoma cell line DAUDI after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 46.1kDa according to NP_000515.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Species ReactivityTested:
Expected from sequence similarity: Human, Mouse, Rat, Dog
Background ReferenceNewman-Tancredi A, Assie MB, Leduc N, Ormiere AM, Danty N, Cosi C.
Novel antipsychotics activate recombinant human and native rat serotonin 5-HT1A receptors: affinity, efficacy and potential implications for treatment of schizophrenia.
Int J Neuropsychopharmacol. 2005 Feb 11;:1-16 [Epub ahead of print]
PMID: 15707540
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Aspiring Grade Product 
Please note, this product is one of a range of Aspiring Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Application Notes below). However no additional claims are made for their ability to recognise native protein in any application. |
