EB06751-T Goat Anti-ERK1 / MAPK3 Antibody - Trial Size
Price: $100.00 / €100,00 / £75.00
Size: 20µg specific antibody in 40µl
EU Availability: In stock
US Availability: In stock
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EB06751 Goat Anti-ERK1 / MAPK3 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
Click to add to EURO Shopping Cart (EU customers only)
Click to add to US Dollar Shopping Cart (US customers only)
Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: MAPK3; ERK1; mitogen-activated protein kinase 3 ; HGNC:6877; P44ERK1; P44MAPK; PRKM3; protein kinase, mitogen-activated 3 (MAP kinase 3 p44)
Official Symbol: MAPK3
Accession Number(s): NP_002737.1
Human GeneID(s): 5595
Important Comments: No cross-reactivity expected with ERK2 Immunogen Peptide with sequence GGEPRRTEGVGP-C, from the N Terminus of the protein sequence according to NP_002737.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 45kDa band observed in Human Brain lysates, Kidney fibroblast HEK293 lysates and epidermoid fibroblast A431 lysates (calculated MW of 43.1kDa according to NP_002737.1). Recommended concentration: 0.1-0.3µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human
Background ReferenceWang PY, Weng J, Anderson RG.
OSBP is a cholesterol-regulated scaffolding protein in control of ERK 1/2 activation.
Science. 2005 Mar 4;307(5714):1472-6.
PMID: 15746430
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06751 (0.3µg/ml) staining of 293 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing ERK1 (RC204196) and probed with EB06751 (mock transfection in second lane), tested by Origene |
