EB06747 Goat Anti-Renalase (aa 224 to 233) Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100ug specific antibody in 200ul
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: C10orf59; chromosome 10 open reading frame 59 ; HGNC:25641; FLJ11218 ; hypothetical protein LOC55328; renalase
Official Symbol: C10orf59
Accession Number(s): NP_001026879.1; NP_060833.1
Human GeneID(s): 55328
Important Comments: This antibody is expected to recognise both reported isoforms (NP_001026879.1and NP_060833.1). Immunogen Peptide with sequence C-VSIDNKKRNI, from the internal region of the protein sequence according to NP_001026879.1; NP_060833.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 37kDa band observed in Human Kidney and Mouse Kidney lysates (calculated MW of 37.8kDa according to NP_001026879.1 and 34.9kDa according to NP_060833.1). Recommended concentration: 0.1-0.3µg/ml.
Immunohistochemistry: In paraffin embedded Human Kidney, stains cytoplasm in cells of renal tubules and in rare cells in the glomeruli. Recommended concentration, 3µg/ml.Species ReactivityTested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog
Background ReferenceXu J, Li G, Wang P, Velazquez H, Yao X, Li Y, Wu Y, Peixoto A, Crowley S, Desir GV.
Renalase is a novel, soluble monoamine oxidase that regulates cardiac function and blood pressure.
J Clin Invest. 2005 May 2;115(5):1275-1280. Epub 2005 Apr 7.
PMID: 15841207
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06747 (1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06747 (3µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. |
