EB06716 Goat Anti-CHMP5 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: CHMP5; chromatin modifying protein 5 ; HGNC:26942; C9orf83; CGI-34; HSPC177; PNAS-2; SNF7DC2; SNF7 domain containing 2; chromosome 9 open reading frame 83
Official Symbol: CHMP5
Accession Number(s): NP_057494.2
Human GeneID(s): 51510
Gene Ontology Terms: HIV infection, MVB sorting
Immunogen Peptide with sequence C-NKDGVLVDEFGLPQ, from the C Terminus of the protein sequence according to NP_057494.2. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 28kDa band observed in lysates of K562 (calculated MW of 24.5kDa according to NP_057494.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ward et al, J Biol Chem. 2005 Mar 18;280(11): 10548-55.; PMID: 15644320). Recommended concentration: 1-3µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human
Background ReferenceWard DM, Vaughn MB, Shiflett SL, White PL, Pollock AL, Hill J, Schnegelberger R, Sundquist WI, Kaplan J.
The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells.
J Biol Chem. 2005 Mar 18;280(11):10548-55. Epub 2005 Jan 11.
PMID: 15644320
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06716 (xµg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
