EB06695 Goat Anti-CD32 / FCGR2B Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100ug specific antibody in 200ul
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: FCGR2B; CD32; FCG2; FCGR2; IGFR2; Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc gamma RIIb; Fc fragment of IgG, low affinity II, receptor for (CD32)
Official Symbol: FCGR2B
Accession Number(s): NP_003992.3; NP_001002273.1; NP_001002274.1; NP_001002275.1;
Human GeneID(s): 2213
Gene Ontology Terms: IgG binding; immune response; integral to membrane; plasma membrane; receptor activity; signal transduction
Important Comments: This antibody is expected to recognise all four reported isoforms (NP_001002273; NP_001002274; NP_001002275; NP_003992) Immunogen Peptide with sequence C-PDALEEPDDQNRI, from the C Terminus of the protein sequence according to NP_003992.3; NP_001002273.1; NP_001002274.1; NP_001002275.1;. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 34kDa band observed in Human myeloid erythroblast cell line K562 and Human monocytic cell line U937 lysates (calculated MW of 34.0kDa according to NP_003992.3;). In transfected HEK293 transiently expressing CD32 a band of approx. 40kDa (glycoprotein) is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human
Background ReferenceMcGaha TL, Sorrentino B, Ravetch JV.
Restoration of tolerance in lupus by targeted inhibitory receptor expression.
Science. 2005 Jan 28;307(5709):590-3.
PMID: 15681388
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06695 (0.3µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing CD32 (RC211982) and probed with EB06695 (mock transfection in first lane), tested by Origene. |
