EB06689 Goat Anti-MEPI / SERPINI2 Antibody Price: $285.00 / €292,50 / £195.00 Size: 100ug specific antibody in 200ul EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: SERPINI2; MEPI; PI14; PANCPIN; TSA2004; SERPIN12; serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 2 Official Symbol: SERPINI2 Accession Number(s): NP_006208.1; NP_001012303.1 Human GeneID(s): 5276 Non-Human GeneID(s): 67931 (mouse); 171149 (rat); Important Comments: This antibody is expected to recognise both reported isoforms (NP_006208.1; NP_001012303.1). Immunogen Peptide with sequence C-KGDWKQKFRKEDTQ, from the internal region of the protein sequence according to NP_006208.1; NP_001012303.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 40kDa band observed in Human Pancreas lysates (calculated MW of 46.1kDa according to NP_006208.1 and 47.4kDa according to NP_001012303.1). Recommended concentration: 0.3-1µg/ml. Immunohistochemistry: In paraffin embedded Human Pancreas, shows cytoplasmic staining of acinar cels and of rare cells in the islets of Langerhans. Recommended concentration, 3µg/ml.Species ReactivityTested: Human Expected from sequence similarity: Human, Mouse, Rat, Dog, Chimpanzee Background ReferenceOzaki K, Nagata M, Suzuki M, Fujiwara T, Miyoshi Y, Ishikawa O, Ohigashi H, Imaoka S, Takahashi E, Nakamura Y. Isolation and characterization of a novel human pancreas-specific gene, pancpin, that is down-regulated in pancreatic cancer cells. Genes Chromosomes Cancer. 1998 Jul;22(3):179-85. PMID: 9624529
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For Research purposes only. Not for diagnostic or in vivo use. © Copyright Everest Biotech Ltd 2009 | Elite Grade Product 

EB06689 (0.3µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

EB06689 (3µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining |