EB06672 Goat Anti-RARA Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: RARA; RAR; NR1B1; retinoic acid receptor, alpha; Retinoic acid receptor, alpha polypeptide; nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form; nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR short form
Official Symbol: RARA
Accession Number(s): NP_000955.1; NP_001019980.1; NP_001028775.1
Human GeneID(s): 5914
Gene Ontology Terms: nucleus; regulation of transcription, DNA-dependent; retinoic acid receptor activity; signal transduction; steroid hormone receptor activity; transcription coactivator activity; transcription factor activity
Important Comments: NP_000955.1 (isfa) and NP_001028775.1(alpha) represent identical proteins. This antibody is expected to recognize all three reported isoforms (NP_000955.1; NP_001019980.1; NP_001028775.1). Please note the epitope is from the mouse/rat sequence. Human sequence is SPSLSPSSNRSSPATHSP and thus highly similar. Immunogen Peptide with sequence C-SPSLSPSSHRSSPATQSP, from the C Terminus of the protein sequence according to NP_000955.1; NP_001019980.1; NP_001028775.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 50+48kDa bands observed in Human Brain lysates (calculated MW of 50.8kDa according to NP_000955.1, NP_001019980.1 and NP_001028775.1). Recommended concentration: 0.03-0.3µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse, Rat
Background ReferenceFarias EF, Ong DE, Ghyselinck NB, Nakajo S, Kuppumbatti YS, Mira y Lopez R.
Cellular retinol-binding protein I, a regulator of breast epithelial retinoic acid receptor activity, cell differentiation, and tumorigenicity.
J Natl Cancer Inst. 2005 Jan 5;97(1):21-9.
PMID: 15632377
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06672 staining (0.03µg/ml) of Human Brain lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
