EB06670 Goat Anti-Asporin / ASPN Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100ug specific antibody in 200ul
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: ASPN; PLAP1; SLRR1C; FLJ20129; asporin (LRR class 1); small leucine-rich protein 1C; periodontal ligament associated protein 1
Official Symbol: ASPN
Accession Number(s): NP_060150.3
Human GeneID(s): 54829
Gene Ontology Terms: extracellular matrix (sensu Metazoa)
Immunogen Peptide with sequence C-IHENKVKKIQKDT, from the internal region of the protein sequence according to NP_060150.3. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 40kDa band observed in Human Bone Marrow lysates (calculated MW of 43.4kDa according to NP_060150.3). Recommended concentration: 1-3µg/ml.
Immunofluoresence in cell line ATDC5 shows signals in cytoplasm and on the cell surface. Data gratefuly received from Dr. Shiro Ikegawa, SNP Research Center, RIKEN, Japan.Species ReactivityTested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat
Background ReferenceKizawa H, Kou I, Iida A, Sudo A, Miyamoto Y, Fukuda A, Mabuchi A, Kotani A, Kawakami A, Yamamoto S, Uchida A, Nakamura K, Notoya K, Nakamura Y, Ikegawa S.
An aspartic acid repeat polymorphism in asporin inhibits chondrogenesis and increases susceptibility to osteoarthritis.
Nat Genet. 2005 Feb;37(2):138-144. Epub 2005 Jan 09.
PMID: 15640800
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06670 (0.3µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06670 staining of ATDC5 cells (Panels A and C) and DAPI (panels B and D). Data gratefuly received from Dr. Shiro Ikegawa, SNP Research Center, RIKEN, Japan. |
