EB06661 Goat Anti-SEMA3E Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: SEMA3E; SEMAH; coll-5; M-SEMAH; M-SemaK; KIAA0331; M-sema H; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3E
Official Symbol: SEMA3E
Accession Number(s): NP_036563.1
Human GeneID(s): 9723
Gene Ontology Terms: cell motility; development; extracellular space; membrane; neurogenesis; receptor activity
Immunogen Peptide with sequence C-KPEHYRLPRHTLDS, from the C Terminus of the protein sequence according to NP_036563.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave bands at approx 35kDa and 20kDa in Human Prostate lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 89.2kDa according to NP_036563.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Immunohistochemistry: In paraffin embedded Human Adrenal Medulla shows granular cytoplasmic staining increasing towards the zona reticularis. Recommended concentration, 2-4µg/ml.Species ReactivityTested: Human
Expected from sequence similarity: Human
Background ReferenceGu C, Yoshida Y, Livet J, Reimert DV, Mann F, Merte J, Henderson CE, Jessell TM, Kolodkin AL, Ginty DD.
Semaphorin 3E and plexin-D1 control vascular pattern independently of neuropilins.
Science. 2005 Jan 14;307(5707):265-8. Epub 2004 Nov 18.
PMID: 15550623
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For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06661 (2.5µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
