EB06659 Goat Anti-ACHE Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: ACHE; YT; acetylcholinesterase (YT blood group)
Official Symbol: ACHE
Accession Number(s): NP_000656.1
Human GeneID(s): 43
Gene Ontology Terms: DNA replication; acetylcholine binding; acetylcholine catabolism in synaptic cleft; acetylcholinesterase activity; amyloid precursor protein metabolism; basal lamina; beta-amyloid binding; cell adhesion; cell proliferation; cholinesterase activity; extracellular region; hydrolase activity; membrane; muscle development; neurotransmitter catabolism; positive regulation of protein secretion; protein homodimerization activity; response to wounding; serine esterase activity; synapse; synaptogenesis
Important Comments: This antibody is expected to recognise isoform NP_000656 only (the ubiquitously expressed, hydrophillic form). Immunogen Peptide with sequence QFDHYSKQDRCSDL, from the C Terminus of the protein sequence according to NP_000656.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 70kDa band observed in Human Brain (Cerebellum and Hippocampus) lysates (calculated MW of 67.8kDa according to NP_000656.1). Recommended concentration: 0.3-1µg/ml.
An additional band of 150kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cat, Rabbit
Background ReferenceCottingham MG, Voskuil JL, Vaux DJ.
The intact human acetylcholinesterase C-terminal oligomerization domain is alpha-helical in situ and in isolation, but a shorter fragment forms beta-sheet-rich amyloid fibrils and protofibrillar oligomers.
Biochemistry. 2003 Sep 16;42(36):10863-73.
PMID: 12962511
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For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06659 (0.3µg/ml) staining of Human Brain (Hippocampus) lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence. |
