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Functional Clustering

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  • Everest Biotech Ltd Goat Anti-DYX1C1 (Isoform a) Antibody
    Online Catalogue > 14-3-3 sigma to FZD9 > Goat Anti-DYX1C1 (Isoform a) Antibody

    EB06656 Goat Anti-DYX1C1 (Isoform a) Antibody
    Price: $285.00 / €292,50 / £195.00
    Size: 100µg specific antibody in 200µl
    EU Availability: In stock
    US Availability: In stock
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    Ordering Information

    As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.

    Target Protein

    Principal Names: DYX1C1; DYX1; EKN1; DYXC1; FLJ37882; dyslexia susceptibility 1 candidate 1
    Official Symbol: DYX1C1
    Accession Number(s): NP_570722.2
    Human GeneID(s): 161582
    Non-Human GeneID(s): 67685 (mouse);
    Gene Ontology Terms: binding; nucleus; Dyslexia
    Important Comments: This antibody is expected to recognise only one of the three reported isoforms (NP_570722.2, isoform a).

    Immunogen

    Peptide with sequence C-KIRNVIQGTELKS, from the C Terminus of the protein sequence according to NP_570722.2.

    Purification + Storage

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    Aliquot and store at -20°C. Minimize freezing and thawing.

    Applications Tested

    Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: lysates of COS1 transfected with full length recombinant Human DYX1C1 gave bands at approx 48kDa after 0.1µg/ml antibody staining. In addition,
    a minor band of 24kDa is detected consistent with observations with N-terminal specific antibodies (unpublished data, LoTurco, Connecticut, USA). The 75kDa band is visible in the non-transfected COS1 also (first lane) and is therefore non-specific. We call for caution when this product is used in other assays than Western blot. Data kindly provided by Wang and LoTurco, University of Connecticut, USA.

    Species Reactivity

    Tested: Human
    Expected from sequence similarity: Human, Mouse, Rat, Chimpanzee

    Product Specific Reference

    The goat polyclonal antibody used in this paper was manufactured by us:

    Wang Y et al. DYX1C1 functions in neuronal migration in developing neocortex. Neuroscience. 2006 Dec 1;143(2):515-522. Epub 2006 Sep 20.
    PMID: 16989952

     


    Adobe PDF Version of Datasheet


    For Research purposes only. Not for diagnostic or in vivo use.

    © Copyright Everest Biotech Ltd 2009

    Elite Grade Product

    Elite Grade Antibodies

    EB06656 Goat Anti-DYX1C1 (Isoform a) Antibody

    EB06656 staining (0.1µg/ml) of COS1 cell lysates: untransfected (left lane) and transfected with full length recombinant Human DYX1C1 (right lane). Data kindly provided by Wang and LoTurco, University of Connecticut, USA.

    Online Catalogue > 14-3-3 sigma to FZD9 > Goat Anti-DYX1C1 (Isoform a) Antibody