EB06654 Goat Anti-AKAP9 / AKAP450 / CG-NAP Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: AKAP9; CG-NAP; PRKA9; YOTIAO; AKAP350; HYPERION; KIAA0803; A kinase (PRKA) anchor protein (yotiao) 9; AKAP120-like protein; kinase N-associated protein; A-kinase anchoring protein 450; A-kinase anchor protein, 350kDa; protein kinase A anchoring protein 9; centrosome- and golgi-localized protein
Official Symbol: AKAP9
Accession Number(s): NP_671700.1; NP_005742.4; NP_671714.1
Human GeneID(s): 10142
Gene Ontology Terms: centrosome; cytoskeleton; protein binding; receptor binding; signal transduction; synaptic transmission; transport
Important Comments: This antibody is expected to recognise isoforms 1, 2 and 3 (NP_671700.1; NP_005742.4; NP_671714.1 respectively). The isoforms Yotiao, AKAP350B and AKAP350C are not recognized. Immunogen Peptide with sequence C-SGSTTQFHAGMR, from the C Terminus of the protein sequence according to NP_671700.1; NP_005742.4; NP_671714.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 450kDa band observed in lysates of cell line HepG2 (calculated MW of 454kDa according to NP_ 671700.1). Recommended concentration: 1-3µg/ml.
Immunohistochemistry: The staining pattern observed was identical to that obtained with a rabbit polyclonal antibody specific to AKAP350A (to be submitted to a scientific journal).Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse
Background ReferenceCiampi R, Knauf JA, Kerler R, Gandhi M, Zhu Z, Nikiforova MN, Rabes HM, Fagin JA, Nikiforov YE.
Oncogenic AKAP9-BRAF fusion is a novel mechanism of MAPK pathway activation in thyroid cancer.
J Clin Invest. 2005 Jan;115(1):94-101.
PMID: 15630448
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For Research purposes only. Not for diagnostic or in vivo use.
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