EB06650 Goat Anti-CCL3 and CCL3L1 Antibody
Price: $185.00 / €187,50 / £125.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3; LD78 alpha beta; Small inducible cytokine A3; small inducible cytokine A3 (homologous to mouse Mip-1a); CCL3L1; LD78; 464.2; SCYA3L; G0S19-2; SCYA3L1; D17S1718; LD78BETA; MGC12815; chemokine (C-C motif) ligand 3-like 1; small inducible cytokine A3-like 1, MIP-1aP
Official Symbol: CCL3L1; CCL3
Accession Number(s): NP_002974.1; NP_066286.1
Human GeneID(s): 6348; 6349
Gene Ontology Terms: chemokine activity; chemotaxis; extracellular region; inflammatory response; negative regulation of cell proliferation
Important Comments: Please note this product is expected to recognize the products of 2 different genes which are almost identical. Immunogen Peptide with sequence C-EWVQKYVSDLELSA, from the C Terminus of the protein sequence according to NP_002974.1; NP_066286.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave bands at approx 26+28kDa (doublet) and 40kDa in Human Bone Marrow and Spleen lysates after 0.1µg/ml antibody staining.The 26+28kDa doublet was also found in Human Lymph Node and in Tonsil lysates. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 10.2kDa according to NP_066286.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain these bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Species ReactivityTested:
Expected from sequence similarity: Human, Chimpanzee
Background ReferenceGonzalez E, Kulkarni H, Bolivar H, Mangano A, Sanchez R, Catano G, Nibbs RJ, Freedman BI, Quinones MP, Bamshad MJ, Murthy KK, Rovin BH, Bradley W, Clark RA, Anderson SA, O'connell RJ, Agan BK, Ahuja SS, Bologna R, Sen L, Dolan MJ, Ahuja SK.
The Influence of CCL3L1 Gene-Containing Segmental Duplications on HIV-1/AIDS Susceptibility.
Science. 2005 Jan 6; [Epub ahead of print]
PMID: 15637236
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Aspiring Grade Product 
Please note, this product is one of a range of Aspiring Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Application Notes below). However no additional claims are made for their ability to recognise native protein in any application. |
