EB06636 Goat Anti-Choline acetyltransferase Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: CHAT; CMS1A; CMS1A2; choline acetyltransferase; acetyl CoA:choline O-acetyltransferase
Official Symbol: CHAT
Accession Number(s): NP_065574; NP_066264; NP_066265; NP_066266
Human GeneID(s): 1103
Important Comments: This antibody is expected to recognise both reported isoforms. NP_066264, NP_066265 and NP_066266 are identical proteins representing isoform 1. Immunogen Peptide with sequence CKEKATRPSQGHQP, from the C Terminus of the protein sequence according to NP_065574; NP_066264; NP_066265; NP_066266. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 70kDa band observed in Human Placenta lysates (calculated MW of 70.5kDa according to NP_065574 and NP_066264). Recommended concentration: 0.03-0.3µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human, Pig, Dog, Chimpanzee
Background ReferenceMadziar B, Lopez-Coviella I, Zemelko V, Berse B.
Regulation of cholinergic gene expression by nerve growth factor depends on the phosphatidylinositol-3'-kinase pathway.
J Neurochem. 2005 Feb;92(4):767-79.
PMID: 15686478
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06636 (0.03µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
