EB06630 Goat Anti-AMID / PRG3 Antibody
Price: $185.00 / €187,50 / £125.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: AMID; PRG3; apoptosis-inducing factor (AIF)-like mitochondrion-associated inducer of death; 5430437E11Rik; p53-responsive gene 3; apoptosis-inducing factor (AIF)-homologous mitochondrion-associated inducer of death
Official Symbol: AMID
Accession Number(s): NP_116186
Human GeneID(s): 84883
Gene Ontology Terms: apoptotic mitochondrial changes; chromosome condensation; cytosol; disulfide oxidoreductase activity; electron transport; electron-transferring-flavoprotein dehydrogenase activity; induction of apoptosis; metabolism; mitochondrial outer membrane
Immunogen Peptide with sequence GSQVSVESGALHC, from the N Terminus of the protein sequence according to NP_116186. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Preliminary experiments gave an approx 48-50kDa band in Human Colon and Duodenum lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.5kDa according to NP_116186. The 48-50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Species ReactivityTested:
Expected from sequence similarity: Human
Background ReferenceOhiro Y, Garkavtsev I, Kobayashi S, Sreekumar KR, Nantz R, Higashikubo BT, Duffy SL, Higashikubo R, Usheva A, Gius D, Kley N, Horikoshi N.
A novel p53-inducible apoptogenic gene, PRG3, encodes a homologue of the apoptosis-inducing factor (AIF).
FEBS Lett. 2002 Jul 31;524(1-3):163-71.
PMID: 12135761
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Aspiring Grade Product 
Please note, this product is one of a range of Aspiring Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Application Notes below). However no additional claims are made for their ability to recognise native protein in any application. |
