EB06599 Goat Anti-pan ADH Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: ADH1B; ADH2; alcohol dehydrogenase IB (class I), beta polypeptide; ADH, beta subunit; aldehyde reductase; alcohol dehydrogenase 2; alcohol dehydrogenase 2 (class I), beta polypeptide; alcohol dehydrogenase 1B (class I), beta polypeptide Official Symbol: ADH1A, B, C Accession Number(s): NP_000658.1; NP_000659.2; NP_000660.1 Human GeneID(s): 124; 125; 126 Important Comments: This antibody is expected to recognise the alpha (ADH1A, NP_ 000658), the beta (ADH1B, NP_000659) and gamma (ADH1C, NP_000660) polypeptide variants of human alcohol dehydrogenase. Immunogen Peptide with sequence STAGKVMKCKA, from the N Terminus of the protein sequence according to NP_000658.1; NP_000659.2; NP_000660.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 38kDa band observed in Human Liver lysates (calculated MW of 39.9kDa according to NP_000658.1, NP_000659.2 and 000660.1). Recommended concentration: 0.3-1.5µg/ml.
Species ReactivityTested: Human Expected from sequence similarity: Human, Mouse, Rat Background ReferenceIkuta T, Fujiyoshi T, Kurachi K, Yoshida A. Molecular cloning of a full-length cDNA for human alcohol dehydrogenase. Proc Natl Acad Sci U S A. 1985 May;82(9):2703-7. PMID: 2986130
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For Research purposes only. Not for diagnostic or in vivo use. © Copyright Everest Biotech Ltd 2009 | Elite Grade Product 

EB06599 (0.3µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |