EB06598 Goat Anti-ABAD / HADH2 Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: HADH2; ABAD; ERAB; MHBD; HSD17B10; 17b-HSD10; hydroxyacyl-Coenzyme A dehydrogenase, type II; type 10 17b-HSD; AB-binding alcohol dehydrogenase; type 10 17beta-hydroxysteroid dehydrogenase Official Symbol: HADH2 Accession Number(s): NP_004484 Human GeneID(s): 3028 Gene Ontology Terms: 3-hydroxyacyl-CoA dehydrogenase activity; 7-alpha-hydroxysteroid dehydrogenase activity; lipid metabolism; metabolism; mitochondrion; oxidoreductase activity; plasma membrane Immunogen Peptide with sequence CIRLDGAIRMQP, from the C Terminus of the protein sequence according to NP_004484. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 25kDa band observed in Human Brain lysates (calculated MW of 26.9kDa according to NP_004484). Recommended concentration: 0.3-2µg/ml. A minor band of unknown identity was also consistently observed at appr 38kDa. This band was successfully blocked by incubation with the immunising peptide.
Species ReactivityTested: Human Expected from sequence similarity: Human, Mouse, Rat, Dog, Bull, Zebrafish, Xenopus, Chimp Background ReferenceHe XY, Schulz H, Yang SY. A human brain L-3-hydroxyacyl-coenzyme A dehydrogenase is identical to an amyloid beta-peptide-binding protein involved in Alzheimer's disease. J Biol Chem. 1998 Apr 24;273(17):10741-6. PMID: 9553139
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For Research purposes only. Not for diagnostic or in vivo use. © Copyright Everest Biotech Ltd 2009 | Elite Grade Product 

EB06598 staining (0.3µg/ml) of Human Brain lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |