EB06597 Goat Anti-HSD11B1 / HDL Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: HSD11B1; HDL; 11-DH; HSD11L; MGC13539; 11-beta-HSD1; hydroxysteroid (11-beta) dehydrogenase 1; corticosteroid 11-beta-dehydrogenase isozyme 1
Official Symbol: HSD11B1
Accession Number(s): NP_005516.1; NP_861420.1
Human GeneID(s): 3290
Gene Ontology Terms: Wnt receptor signaling pathway; development; extracellular; growth factor activity; negative regulation of Wnt receptor signaling pathway; signal transducer activity
Important Comments: This antibody is expected to recognise human HSD11B1 protein. Both NP_005516.1 and NP_861420.1 variants encode the same protein. Immunogen Peptide with sequence CTSYNMDRFINK, from the C Terminus of the protein sequence according to NP_005516.1; NP_861420.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 35kDa band observed in Human Liver lysates (calculated MW of 32.4kDa according to NP_005516.1 and NP_861420.1). Recommended concentration: 0.3-2µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human, Chimpanzee, Sheep, Cow
Background ReferencePaterson JM, Morton NM, Fievet C, Kenyon CJ, Holmes MC, Staels B, Seckl JR, Mullins JJ.
Metabolic syndrome without obesity: Hepatic overexpression of 11beta-hydroxysteroid dehydrogenase type 1 in transgenic mice.
Proc Natl Acad Sci U S A. 2004 May 4;101(18):7088-93. Epub 2004 Apr 26.
PMID: 15118095
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06597 (0.3µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
