EB06566 Goat Anti-Prostaglandin dehydrogenase 1 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
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US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: HPGD; PGDH1; 15-PGDH; hydroxyprostaglandin dehydrogenase 15-(NAD); Prostaglandin dehydrogenase 1
Official Symbol: HPGD
Accession Number(s): NP_000851
Human GeneID(s): 3248
Gene Ontology Terms: 15-hydroxyprostaglandin dehydrogenase (NAD+) activity; electron transporter activity; metabolism; oxidoreductase activity; prostaglandin metabolism; prostaglandin-D synthase activity
Immunogen Peptide with sequence C-DYDTTPFQAKTQ, from the C Terminus of the protein sequence according to NP_000851. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: experiments gave bands at approx 26kDa and 50kDa in Human Duodenum lysates after 0.1µg/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (PMID: 11889207). This protein has a calculated MW of 29.0kDa according to NP_000851. Recommended concentration: 0.1-1µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human, Chimpanzee
Background ReferenceCoggins KG, Latour A, Nguyen MS, Audoly L, Coffman TM, Koller BH.
Metabolism of PGE2 by prostaglandin dehydrogenase is essential for remodeling the ductus arteriosus.
Nat Med. 2002 Feb;8(2):91-2. No abstract available.
PMID: 11821873
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06566 (0.1µg/ml) staining of Human Duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
