EB06563 Goat Anti-FLVCR Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: FLVCR; feline leukemia virus subgroup C cellular receptor; FLVCR protein Official Symbol: FLVCR Accession Number(s): NP_054772.1 Human GeneID(s): 28982 Gene Ontology Terms: development; integral to plasma membrane; receptor activity; transport; transporter activity Immunogen Peptide with sequence C-KTVMLSKQSESAI, from the C Terminus of the protein sequence according to NP_054772.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 55kDa band observed in Human Bone Marrow lysates (calculated MW of 59.9kDa according to NP_054772.1). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? EB06563 has been successfully used in WB application in the publicatioon: Rey M.A. et al. Enhanced alternative splicing of the FLVCR1 gene in Diamond Blackfan anemia disrupts FLVCR1 expression and function that are critical for erythropoiesis. Haematologica 2008 Nov;93(11):1617-26. Epub 2008 Sep 24. PMID:18815190.Species ReactivityTested: Human Expected from sequence similarity: Human, Chimpanzee, Cat Product Specific ReferenceThe goat polyclonal antibody used in this paper was manufactured by us: Rey M.A. et al. Enhanced alternative splicing of the FLVCR1 gene in Diamond Blackfan anemia disrupts FLVCR1 expression and function that are critical for erythropoiesis. Haematologica 2008 Nov;93(11):1617-26. Epub 2008 Sep 24. PMID: 18815190
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For Research purposes only. Not for diagnostic or in vivo use. © Copyright Everest Biotech Ltd 2009 | Elite Grade Product 

EB06563 (0.1µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |