EB06538 Goat Anti-ABCC4 / MRP4 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100ug specific antibody in 200ul
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: ABCC4; MRP4; MOATB; MOAT-B; EST170205; ATP-binding cassette, sub-family C (CFTR/MRP), member 4; canalicular multispecific organic anion transporter (ABC superfamily); Multidrug Resistance Protein 4
Official Symbol: ABCC4
Accession Number(s): NP_005836.2
Human GeneID(s): 10257
Non-Human GeneID(s): 170924 (rat);
Gene Ontology Terms: 15-hydroxyprostaglandin dehydrogenase (NAD+) activity; ATP binding; ATPase activity; ATPase activity, coupled to transmembrane movement of substances; chloride channel activity; integral to membrane; ion transport
Important Comments: This antibody is expected to recognize isoform 1 (NP_005836.2) only. Immunogen Peptide with sequence CNGQPSTLTIFETAL, from the C Terminus of the protein sequence according to NP_005836.2. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 170kDa band observed in rat renal cortex membranes (calculated MW of 149kDa according to Rat NP_596902.1 and 150kDa according to Human NP_005836.2). Data kindly provided by Dr. Antje Steinbach, Ernst-Moritz-Arndt University of Greifswald, Germany. Recommended concentration: 1-3µg/ml.
Immunohistochemistry: In paraffin embedded Human Prostate shows strong cytoplasmic staining in small flat basal cells and weak cytoplasmic staining in the columnar secretory cells of the epithelium. Recommended concentration, 2-4µg/ml.Species ReactivityTested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat
Background ReferenceSchuetz JD, Connelly MC, Sun D, Paibir SG, Flynn PM, Srinivas RV, Kumar A, Fridland A.
MRP4: A previously unidentified factor in resistance to nucleoside-based antiviral drugs.
Nat Med. 1999 Sep;5(9):1048-51.
PMID: 10470083
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06538 staining (0.5µg/ml) of rat renal cortex membranes (20µg total protein per lane). Detected by western blot using chemiluminescence. Data kindly provided by Dr. Antje Steinbach, Ernst-Moritz-Arndt University of Greifswald.
EB06538 (2.5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
