EB06536 Goat Anti-ADAM12 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: ADAM12; MCMP; MLTN; MLTNA; MCMPMltna; a disintegrin and metalloproteinase domain 12 (meltrin alpha); meltrin alpha; A disintegrin and metalloproteinase domain 12 (Meltrin-alpha, mouse, homolog of)
Official Symbol: ADAM12
Accession Number(s): NP_003465; NP_067673
Human GeneID(s): 8038
Gene Ontology Terms: cell adhesion; hydrolase activity; integral to membrane; metalloendopeptidase activity; myoblast fusion; plasma membrane; protein binding; proteolysis and peptidolysis; zinc ion binding
Important Comments: This antibody is expected to recognise both the longer membrane-bound form of human ADAM12 (NP_003465) and the shorter soluble ADAM12 splice isoform (NP_067673). This antibody does not cross-react with other ADAMS. Immunogen Peptide with sequence AARPLPVSPARALC, from the N Terminus of the protein sequence according to NP_003465; NP_067673. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 90kDa band observed in human heart lysates (calculated MW of 99.6kDa according to NP_003465 and 80.5kDa according to NP_067673). Recommended concentration: 0.1-0.3µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human
Background ReferenceGalliano MF, Huet C, Frygelius J, Polgren A, Wewer UM, Engvall E.
Binding of ADAM12, a marker of skeletal muscle regeneration, to the
muscle-specific actin-binding protein, alpha -actinin-2, is required for
myoblast fusion.
J Biol Chem. 2000 May 5;275(18):13933-9.
PMID: 10788519
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06536 staining (0.1µg/ml) of human heart lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
