EB06532 Goat Anti-CREM Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: CREM; ICER; MGC17881; MGC41893; MGC111110; hCREM-2; cAMP responsive element modulator; hCREM 2alpha-b protein; hCREM 2beta-a protein; inducible cAMP early repressor ICER
Official Symbol: CREM
Accession Number(s): NP_853549.1; NP_874387.1; NP_874388.1; NP_874393.1; NP_874394.1; NP_877570.1; NP_877571.1; NP_877572.1; NP_877573.1; NP_878270.1; NP_898883.1
Human GeneID(s): 1390
Gene Ontology Terms: DNA binding; cAMP response element binding protein binding; nucleus; nucleus; nucleus; protein binding; regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; signal transduction; transcription factor activity
Important Comments: This antibody is expected to recognise the following protein isoforms of human CREM protein: a (NP_853549.1); e (NP_874387.1); f (NP_874388.1); k (NP_874393.1); l (NP_874394.1); m (NP_877570.1); n (NP_877571.1); o (NP_877572.1); p (NP_877573.1); q (NP_878270.1); v (NP_898883.1). This antibody is not expected to cross-react with CREB protein. Immunogen Peptide with sequence LETLKDICSPKTDY, from the C Terminus of the protein sequence according to NP_853549.1; NP_874387.1; NP_874388.1; NP_874393.1; NP_874394.1; NP_877570.1; NP_877571.1; NP_877572.1; NP_877573.1; NP_878270.1; NP_898883.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 30kDa band observed in human heart lysates (calculated MW of 32.5kDa according to NP_853549.1). Recommended concentration: 1-3µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse, Rat
Background ReferenceTenbrock K, Juang YT, Tolnay M, Tsokos GC.
The cyclic adenosine 5'-monophosphate response element modulator suppresses IL-2 production in stimulated T cells by a chromatin-dependent mechanism.
J Immunol. 2003 Mar 15;170(6):2971-6.
PMID: 12626549
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06532 staining (1µg/ml) of human heart lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
