EB06530 Goat Anti-PRDM4 / PFM1 Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: PRDM4; PFM1; MGC45046; PR domain containing 4; PR-domain zinc-finger protein PFM1 Official Symbol: PRDM4 Accession Number(s): NP_036538 Human GeneID(s): 11108 Gene Ontology Terms: DNA binding; RNA polymerase II transcription factor activity; cell proliferation; nucleus; regulation of transcription, DNA-dependent; signal transduction; transcription from Pol II promoter; zinc ion binding Immunogen Peptide with sequence CSAVYSADESLSAHK, from the C Terminus of the protein sequence according to NP_036538. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 70kDa band observed in human and mouse brain lysates (calculated MW of 87.9kDa according to NP_036538). Recommended concentration: 0.3-2µg/ml.
Species ReactivityTested: Human, Mouse Expected from sequence similarity: Human, Mouse, Rat Background ReferenceChittka A, Arevalo JC, Rodriguez-Guzman M, Perez P, Chao MV, Sendtner M. The p75NTR-interacting protein SC1 inhibits cell cycle progression by transcriptional repression of cyclin E. J Cell Biol. 2004 Mar 29;164(7):985-96. PMID: 15051733
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use. © Copyright Everest Biotech Ltd 2009 | Elite Grade Product 

EB06530 (0.3µg/ml) staining of human brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |