EB06526 Goat Anti-RXR gamma Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: RXRG; RXRC; NR2B3; retinoid X receptor, gamma
Official Symbol: RXRG
Accession Number(s): NP_008848
Human GeneID(s): 6258
Gene Ontology Terms: nucleus; regulation of transcription, DNA-dependent; retinoid-X receptor activity; steroid binding; steroid hormone receptor activity; transcription factor activity
Important Comments: This antibody is expected to recognise an epitope corresponding to aa 213-226 of human RXR gamma protein (hinge region) and does not cross-react with either RXR alpha or beta. Immunogen Peptide with sequence RQRSRERAESEAEC, from the internal region of the protein sequence according to NP_008848. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 48kDa band observed in human brain lysates (calculated MW of 50.9kDa according to NP_008848). Recommended concentration: 0.3-2µg/ml. Some bands of unknown identity were also consistently observed around 37 kDa. These bands were successfully blocked by incubation with the immunising peptide.
Species ReactivityTested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig
Background ReferenceSamad TA, Krezel W, Chambon P, Borrelli E.
Regulation of dopaminergic pathways by retinoids: activation of the D2 receptor promoter by members of the retinoic acid receptor-retinoid X receptor family.
Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14349-54.
PMID: 9405615
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06526 (0.3µg/ml) staining of human brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
