EB06450 Goat Anti-Smooth muscle alpha-actin Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: Currently out of stock - Please email sales@everestbiotech.com for delivery estimate US Availability: Out of stock - If product is in stock in the EU then please allow up to 10 days for delivery Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: ACTA2; ACTSA; actin, alpha 2, smooth muscle, aorta; AAT6; ACTSA; OTTHUMP00000020042; alpha 2 actin; alpha-cardiac actin; growth-inhibiting gene 4; smooth muscle alpha-actin; Official Symbol: ACTA2 Accession Number(s): NP_001604.1 Human GeneID(s): 59 Gene Ontology Terms: actin filament; motor activity; muscle development; striated muscle thin filament; structural constituent of cytoskeleton; structural constituent of muscle Immunogen Peptide with sequence EEEDSTALVC, from the N Terminus of the protein sequence according to NP_001604.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:1,000. Western Blot: Approx 45kDa band observed in Human Duodenum and Human Heart lysates (calculated MW of 42.0kDa according to NP_001604.1). Recommended concentration: 0.1-0.3µg/ml.
Species ReactivityTested: Human Expected from sequence similarity: Human, Mouse, Rat, Dog Product Specific ReferenceThe goat polyclonal antibody used in this paper was manufactured by us: McIntire et al.: Novel HLA-G-binding leukocyte immunoglobulin-like receptor (LILR) expression patterns in human placentas and umbilical cords. Placenta. 2008 Jul;29(7):631-8. Epub 2008 Jun 5. PMID: 18538388
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For Research purposes only. Not for diagnostic or in vivo use. © Copyright Everest Biotech Ltd 2009 | Elite Grade Product 

EB06450 staining (0.1µg/ml) of Human Duodenum lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |