EB06448 Goat Anti-Survivin / BIRC5 Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
Click to add to EURO Shopping Cart (EU customers only)
Click to add to US Dollar Shopping Cart (US customers only)
Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: BIRC5; API4; EPR-1; baculoviral IAP repeat-containing 5 (survivin); survivin; apoptosis inhibitor 4
Official Symbol: BIRC5
Accession Number(s): NP_001159.2; NP_001012271.1
Human GeneID(s): 332
Gene Ontology Terms: 3-oxo-5-alpha-steroid 4-dehydrogenase activity; DNA repair; androgen metabolism; cell-cell signaling; electron transporter activity; integral to membrane; male gonad development; methylated-DNA-[protein]-cysteine S-methyltransferase activity; microsome; oxidoreductase activity; sex determination; sex differentiation
Important Comments: This antibody is expected to recognize the reported isoforms 1 and 3 (NP_001159.2 and NP_001012271.1 resp.) Immunogen Peptide with sequence CVRRAIEQLAAMD, from the C Terminus of the protein sequence according to NP_001159.2; NP_001012271.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 18kDa band observed in Human T cell line MOLT4 lysates (calculated MW of 16.4kDa according to NP_001159.2 and 18.6kDa according to NP_001012271.1). Recommended concentration: 0.01-0.5µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human
Background ReferenceLi F, Ambrosini G, Chu EY, Plescia J, Tognin S, Marchisio PC, Altieri DC.
Control of apoptosis and mitotic spindle checkpoint by survivin.
Nature. 1998 Dec 10;396(6711):580-4.
PMID: 9859993
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06448 (0.01µg/ml) staining of Molt-4 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
