EB06444-T Goat Anti-BCL2 Antibody - Trial Size Price: $100.00 / €100,00 / £75.00 Size: 20µg specific antibody in 40µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info EB06444 Goat Anti-BCL2 Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: BCL2; Bcl-2; B-cell CLL/lymphoma 2 Official Symbol: BCL2 Accession Number(s): NP_000624; NP_000648 Human GeneID(s): 596 Non-Human GeneID(s): 12043 (mouse); 24224 (rat); Gene Ontology Terms: anti-apoptosis; cell growth and/or maintenance; humoral immune response; integral to membrane; mitochondrial outer membrane; mitochondrion; negative regulation of cell proliferation; protein binding; regulation of apoptosis; regulation of cell cycle Important Comments: This antibody is expected to recognise both reported isoforms (NP_000624; NP_000648) Immunogen Peptide with sequence AGRTGYDNREIVMKYC, from the N Terminus of the protein sequence according to NP_000624; NP_000648. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 25 kDa band observed in Human Lymph node lysates (predicted MW of 26kDa according to NP_000624, alpha isoform). Recommended for use at 1-3µg/ml.
Species ReactivityTested: Human Expected from sequence similarity: Human, Mouse, Rat Product Specific ReferenceThe goat polyclonal antibody used in this paper was manufactured by us: Yu X.Q. et al.: Tanshinone IIB, a primary active constituent from Salvia miltiorrhiza, exerts neuroprotective effect via inhibition of neuronal apoptosis in vitro. Phytother Res. 2008 Jun;22(6):846-50. PMID: 18389485 Yu X.Q. et al.: In vitro and in vivo neuroprotective effect and mechanisms of glabridin, a major active isoflavan from Glycyrrhiza glabra (licorice). Life Sci. 2008 Jan 2;82(1-2):68-78. Epub 2007 Nov 7. PMID: 18048062
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EB06444 staining (1µg/ml) of Human Lymph Node lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |