EB06437 Goat Anti-Pirin Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: PIR; pirin (iron-binding nuclear protein); Pirin Official Symbol: PIR Accession Number(s): NP_003653 Human GeneID(s): 8544 Gene Ontology Terms: nucleus; transcription cofactor activity; transcription from Pol II promoter Immunogen Peptide with sequence GSSKKVTLSVLSREC, from the N Terminus of the protein sequence according to NP_003653. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 30-35kDa band observed in Human Heart lysate (calculated MW of 32.1kDa according to NP_003653. Recommended concentration: 0.3 - 1.0 µg/ml.
Species ReactivityTested: Human Expected from sequence similarity: Human, Mouse Background ReferenceWendler WM, Kremmer E, Forster R, Winnacker EL. Identification of pirin, a novel highly conserved nuclear protein. J Biol Chem. 1997 Mar 28;272(13):8482-9. PMID: 9079676
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For Research purposes only. Not for diagnostic or in vivo use. © Copyright Everest Biotech Ltd 2009 | Elite Grade Product 

EB06437 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |