EB06389 Goat Anti-Lime1 (mouse) Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: Lime1; Lck interacting transmembrane adaptor 1; 2810038K19Rik; LIME; RIKEN cDNA 2810038K19 gene
Official Symbol: Lime1
Accession Number(s): NP_076173.1
Human GeneID(s):
Non-Human GeneID(s): 72699 (mouse);
Gene Ontology Terms: integral to membrane; extracellular space
Immunogen Peptide with sequence C-ENVYESIKEMGL, from the C Terminus of the protein sequence according to NP_076173.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution >1:128,000. Western Blot: Approx 32kDa band observed in Mouse Heart lysates (predicted MW of 31kDa according to NP_076173.1). Recommended for use at 0.5-2µg/ml.
Species ReactivityTested: Mouse
Expected from sequence similarity: Mouse, Rat
Background ReferenceHur EM, Son M, Lee OH, Choi YB, Park C, Lee H, Yun Y.
LIME, a novel transmembrane adaptor protein, associates with p56lck and mediates T cell activation.
J Exp Med. 2003 Nov 17;198(10):1463-73. Epub 2003 Nov 10.
PMID: 14610044
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06389 staining (1µg/ml) of Mouse Heart lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
