EB06377-T Goat Anti-GAPDH (C Terminus) Antibody - Trial Size
Price: $100.00 / €100,00 / £75.00
Size: 20µg specific antibody in 40µl
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US Availability: In stock
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EB06377 Goat Anti-GAPDH (C Terminus) Antibody
Price: $300.00 / €300,00 / £205.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
Click to add to EURO Shopping Cart (EU customers only)
Click to add to US Dollar Shopping Cart (US customers only)
Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: GAPDH; glyceraldehyde-3-phosphate dehydrogenase; HGNC:4141; G3PD; GAPD; MGC88685; OTTHUMP00000174431; OTTHUMP00000174432; aging-associated gene 9 protein; glyceraldehyde 3-phosphate dehydrogenase
Official Symbol: GAPDH
Accession Number(s): NP_002037.2
Human GeneID(s): 2597
Gene Ontology Terms: glucose metabolism; glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; oxidoreductase activity;
Important Comments: GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting. Immunogen Peptide with sequence C-HQVVSSDFNSDT, from the C Terminus of the protein sequence according to NP_002037.2. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:2000.
Western Blot: Approx 35kDa band observed in lysates of Human Tonsil, Mouse Spleen, and cell lines HeLa, A431 and 293 (calculated MW of 36.1kDa according to NP_002037). Recommended concentration: 0.01-0.03µg/ml.
Immunohistochemistry: In paraffin embedded Human Tonsil, staining of cells in germinal centre, mantle zone, interfollicular area, epithelium and endothelium is observed. Recommended concentration 0.3ug/ml. (IHC data have been obtained with EB06377 from previous batch).
Species ReactivityTested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Dog
Product Specific ReferenceThe goat polyclonal antibody used in this paper was manufactured by us:
Kiepe D. et al.: Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein 2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I.
Endocrinology. 2008 Oct;149(10):4901-11. Epub 2008 Jun 12.
PMID: 18556354
Carr AJ et al., Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay. Mol Vis. 2009;15:283-95. Epub 2009 Feb 6.
PMID: 19204785
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For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06377 staining (0.01µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB06377 (0.3µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Staining of germinal centre cells. B) Staining of endothelial cells. |
