EB06354 Goat Anti-ARPC3 Antibody Price: $285.00 / €292,50 / £195.00 Size: 100µg specific antibody in 200µl EU Availability: In stock US Availability: In stock Click to add to EURO Shopping Cart (EU customers only) Click to add to US Dollar Shopping Cart (US customers only) Cart for UK (GBP) customers coming soon, meanwhile click for ordering info
Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: ARPC3; ARC21; p21-Arc; actin related protein 2/3 complex, subunit 3, 21kDa; ARP2/3 protein complex subunit p21 Official Symbol: ARPC3 Accession Number(s): NP_005710 Human GeneID(s): 10094 Non-Human GeneID(s): 56378 (mouse); Gene Ontology Terms: structural constituent of cytoskeleton; cell motility; Arp2/3 protein complex; actin cytoskeleton Important Comments: Please note there are two hypothetical proteins called "similar to the Arp2/3 protein complex subunit p21-Arc" that are virtually identical (CAC14083, XP_208062). Immunogen Peptide with sequence C-RQFMNKSLSGPGQ, from the C Terminus of the protein sequence according to NP_005710. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Band observed just below 20kDa marker in Hela, Human Brain and Mouse Brain lysates (predicted MW of 20kDa according to NP_005710). Recommended for use at 1-3µg/ml.
Species ReactivityTested: Human, Mouse Expected from sequence similarity: Human, Mouse, Rat Background ReferenceWelch MD, DePace AH, Verma S, Iwamatsu A, Mitchison TJ. The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. J Cell Biol. 1997 Jul 28;138(2):375-84. PMID: 9230079
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For Research purposes only. Not for diagnostic or in vivo use. © Copyright Everest Biotech Ltd 2009 | Elite Grade Product 

EB06354 staining (1µg/ml) of Mouse Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |