EB06333 Goat Anti-AKAP10 / D-AKAP2 Antibody
Price: $185.00 / €187,50 / £125.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: AKAP10; PRKA10; D-AKAP2; MGC:9414; A kinase (PRKA) anchor protein 10; dual specificity A-Kinase anchoring protein 2
Official Symbol: AKAP10
Accession Number(s): NP_009133
Human GeneID(s): 11216
Non-Human GeneID(s): 56697 (mouse);
Gene Ontology Terms: protein binding activity; protein localization; signal transduction
Immunogen Peptide with sequence C-AQYDQPLEKSTKL, from the C Terminus of the protein sequence according to NP_009133. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave no signal but low background in Human Skeletal Muscle lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Species ReactivityTested:
Expected from sequence similarity: Human, Mouse
Background ReferenceHuang LJ, Durick K, Weiner JA, Chun J, Taylor SS.
D-AKAP2, a novel protein kinase A anchoring protein with a putative RGS domain.
Proc Natl Acad Sci U S A. 1997 Oct 14;94(21):11184-9.
PMID: 9326583
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Aspiring Grade Product 
Please note, this product is one of a range of Aspiring Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Application Notes below). However no additional claims are made for their ability to recognise native protein in any application. |
