EB06330 Goat Anti-MPG Antibody
Price: $285.00 / €292,50 / £195.00
Size: 100µg specific antibody in 200µl
EU Availability: In stock
US Availability: In stock
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Ordering Information As well as the shopping cart links above, you can order by email, phone, fax and mail. For country specific ordering information and shopping cart instructions please see Ordering Info. Please get in touch for world-beating prices on bulk orders and custom formulations. To order the immunizing peptide for blocking experiments please see Peptide Info.Target ProteinPrincipal Names: N-methylpurine-DNA glycosylase; AAG; APNG; CRA36.1; MDG; Mid1; N-methylpurine-DNA glycosylase, MPG; PIG11; PIG16; anpg; 3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on 16p; CRA36.1 (3-methyl-adenine DNA glycosylase); alkyladenine DNA glycosylase; proliferation-inducing protein 11; proliferation-inducing protein 16
Official Symbol: MPG
Accession Number(s): NP_002425.2; NP_001015052.1; NP_001015054.1
Human GeneID(s): 4350
Gene Ontology Terms: damaged DNA binding activity; DNA glycolase; DNA dealkylation; base-excision repair; nucleoplasm
Important Comments: This antibody is expected to recognize all three reported isoforms (NP_002425.2, NP_001015052.1 and NP_001015054.1) Immunogen Peptide with sequence C-SVVDRVAEQDTQA, from the C Terminus of the protein sequence according to NP_002425.2; NP_001015052.1; NP_001015054.1. Purification + Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.Applications TestedPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 28-30kDa band observed in lysates of cell line K562 (calculated MW of 30.8kDa according to NP_001015054.1). Recommended concentration: 1-3µg/ml.
Species ReactivityTested: Human
Expected from sequence similarity: Human
Background ReferenceSamson L, Derfler B, Boosalis M, Call K.
Cloning and characterization of a 3-methyladenine DNA glycosylase cDNA from human cells whose gene maps to chromosome 16.
Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9127-31.
PMID: 1924375
Adobe PDF Version of Datasheet
For Research purposes only. Not for diagnostic or in vivo use.
© Copyright Everest Biotech Ltd 2009 | Elite Grade Product 
EB06330 staining (2µg/ml) of K562 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
